Broadening the Scope of Hydroxyl Radical Protein Footprinting Applications
ABSTRACT
Professor Sharp discusses advancements in protein footprinting technology, including the ability to perform experiments on benchtops using UV-catalyzed hydroxyl radicals for protein labeling. He explains how this technique can address challenges in studying dynamic, heterogeneous systems like glycoproteins, aggregation processes, and phosphorylation. Applications include studying HIV glycoproteins, malaria proteins, and monoclonal antibody aggregation.
SPEAKER
Dr. Joshua Sharp is an internationally recognized expert in HRPF, pioneering novel MS-based technology to study HOS focusing on the structure-function relationships of proteins and carbohydrates. With more than 21 peer-reviewed articles, Dr. Sharp has also presented numerous invited lectures around the world. Along with acting as the Chief Technology Officer of GenNext, Dr. Sharp is also Associate Professor of Pharmacology, Associate Professor of Chemistry and Biochemistry, and Director of the Glycoscience Center of Research Excellence at the University of Mississippi.
