Next Generation of
Structural Biology Research

Reinventing Protein Higher Order Structural Analysis via State-of-the-Art Protein Footprinting

Fox® Protein Footprinting Technology employs hydroxyl radicals (•OH) to examine the solvent accessibility of amino acid side chains within a protein. By selectively labeling exposed amino acids, •OH radicals induce the formation of oxidation products in exposed amino acids, enabling the detection and analysis of vital information concerning protein structure and dynamics.

Unlike other techniques, Fox Protein Footprinting delivers dynamic, in-solution structure and interaction information on a wide range of protein sizes, states, and concentrations.

Automated Radical Protein Footprinting, made possible by the AutoFox® System, delivers crucial HOS information that greatly influences the safety, efficacy, and quality of biologics.

Comprised of a user-friendly benchtop instrument coupled with intuitive data analysis software, the Fox platform makes it possible for biopharmaceutical researchers to easily and efficiently unravel the mysteries of protein HOS.

This powerful structural biology tool opens new doors to the understanding of proteins, accelerating advancements in biopharmaceutical research and development.

Advantages

  • Direct Assessment of Solvent Accessibility

  • Higher-Order Structure Validation

  • High Spatial Resolution

  • Versatility Across Protein Classes

  • Complementary to Other Techniques

Insights

  • Protein-Protein Interactions

  • Epitope & Paratope Mapping

  • Protein-Ligand Interactions

  • Protein Folding & Conformational Changes

  • Regions Impacted by Allostery

  • Glycoprotein Structure & Function

  • Small Molecule Therapeutic Target Engagement & Response

  • Rapidly Validate AI-Based Structural Models

Hands-Free, High-Throughput & Reproducible Labeling

First Fully-Automated Radical Protein Footprinting

With the launch of the AutoFox Protein Footprinting System, GenNext enables structural biology researchers to perform high-resolution protein footprinting studies with robustness, reproducibility, and ease—generating high-value structural insights in days instead of weeks or months.

GenNext’s FoxWare® Software removes data processing bottlenecks with this powerful program tailored to meet the demands of HOS analysis.

Learn how our products can easily fit into your lab’s workflow, or test-drive the Fox System on an outsourced project basis.

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Structural Biology Services

Outsource Protein Footprinting Studies Starting Today!

Let the experienced GenNext research team generate high-value information on the characterization of your protein for more effective and safe biologics. Start a project today to study protein-protein interaction sites, epitope/paratope mapping, host antibody responses, antibody aggregation, and more.

Complement your traditional HOS methods or take a new route to modern HOS analysis. Use our contract research services to deliver actionable protein characterization data.

Applications from Discovery to Development

Insights from Biosimilars to AI Model Validation

Biotherapeutics & Monoclonal Antibodies

Access structural insights for preclinical drug development within days, not weeks or months.

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Small Molecule Drug Discovery

Promote promising leads while eliminating those that inappropriately bind or elicit an undesired target response.

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Artificial Intelligence & Computational Biology

Enhance the probability of drug discovery success by quickly confirming or refuting AI hypotheses.
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Delivering HOS Answers in a New Way

The Fox Technology platform pairs an intuitive workflow with the robust FoxWare Protein Footprinting Software, producing results that rival even the most advanced, technically demanding, and expensive methods in structural biology. This integrated approach streamlines complex analyses, making top-tier structural data attainable for a wider range of laboratories. By minimizing operational complexity and lowering costs, the system empowers researchers to gain detailed insights into protein structures and dynamics with greater efficiency and ease than ever before.

See how the Fox platform stacks up against other protein HOS analysis methods.

GenNext News

Breaking News
GenNext Technologies and Thermo Fisher Scientific Collaborate to Simplify Structural Biology for Faster Biotherapeutic Development
GenNext and Agilent Unveil Groundbreaking Technology Integration to Accelerate High-Value Decision Making
Evaluating MS-Based HRPF of a Benchtop Fox System against a Synchrotron X-ray Beamline
GenNext Unveils the First Fully Automated HRPF System at the CASSS MS Meeting
GenNext & SCIEX to Co-Market an Advanced HOS Workflow for Biopharmaceutical Research
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GenNext & Thermo Fisher Collaborate to Simplify Structural Biology

GenNext's AutoFox System and Thermo Scientific™ instrumentation platform speeds biotherapeutic development and supports AI in drug discovery.

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GenNext and Agilent Unveil Groundbreaking Technology Integration

Advancing high-resolution structural biology research with GenNext's AutoFox Protein Footprinting System and the Agilent 6500 Series of Quadrupole Time-of-Flight LC/MS systems.

Featured Scientific Presentations

The GenNext AutoFox® Protein Footprinting System combined with mass spectrometry enables amino acid-level mapping of protein interactions and conformational changes. Advanced MS tools like the Sciex ZenoTOF 7600 and Agilent 6560 address challenges include fragment ion identification for accurate oxidation localization and isobaric peptide co-elution causing chimeric spectra. Furthermore, integrating Thermo’s XL-MS further resolves ambiguous signals, advancing biologics and biotherapeutics research.

Presented by Dr. Emily Chea, Applied Research Manager of GenNext Technologies, Inc.

The AutoFox® Protein Footprinting System is a mass spectrometry-enabled platform for mapping protein structures and detecting interaction sites by oxidizing solvent-accessible amino acids. As structural changes or protein interactions affect solvent accessibility, modification levels shift accordingly. The AutoFox System is coupled with bottom-up proteomics and reverse-phase chromatography, to localize and quantify the extent of oxidation. Ion mobility separation further enhances resolution by resolving co-eluted, isobaric peptides before MS/MS fragmentation, improving residue-level precision.

Presented by Dr. Emily Chea, Applied Research Manager of GenNext Technologies, Inc

Many structural biology researchers seek easier and more robust methods for Higher Order Structural (HOS) analysis, recognizing its critical role in biotherapeutic stability, safety, and function. Traditional HOS approaches can be complex, slow, dangerous, and costly, or unreliable and uninformative. With many factors to consider, choosing the best method for your lab can be challenging. Dr. Chea reviews various options, highlighting their pros and cons, and introduces the Flash Oxidation (Fox®) Protein Footprinting System. She explains its applications in epitope mapping, mAb aggregation, and conformational analysis, helping you select the best method for developing safe and effective biotherapeutics.

Presented by Dr. Emily Chea, Applied Research Manager of GenNext Technologies, Inc.

Dr. Sharp discusses a covalent labeling technique called fast photochemical oxidation of proteins (FPOP), detailing the workflow, its application in research, and how a new platform is designed to enhance user-friendliness. The Fox Oxidation System streamlines the entire process, from radical generation to sample collection, improving safety, reliability, and ease of use compared to traditional FPOP setups. This innovation makes HRPF accessible to more researchers, allowing them to gain valuable insights into protein structures and interactions with greater ease.

Presented by Dr. Joshua Sharp, Research Institute of Pharmaceutical Sciences at the University of Mississippi.

This hour-long seminar, hosted with The Analytical Scientist, reviews structural biology case studies showcasing the successful application of protein footprinting in solving key problems in biopharmaceutical research. Examples include epitope and paratope mapping, protein interactions, receptor-drug interactions, and protein folding/unfolding. The seminar highlights the benefits of using protein footprinting technology in advancing biopharmaceutical research.

Presented by Professor Michael Gross of the Washington University in St. Louis.

A study on TNF alpha and its monoclonal antibody adalimumab, using the Fox Protein Footprinting System, revealed oxidation changes in TNF alpha peptides matching the known epitope. Adalimumab’s Fab region showed decreased oxidation due to reduced solvent accessibility when bound to TNF alpha, while the Fc region had increased oxidation, indicating flexibility beyond the crystal structure. This method accurately mapped the TNF alpha epitope and adalimumab paratope, providing insights beyond the crystal structure.

Presented by Dr. Emily Chea, Applied Research Manager of GenNext Technologies, Inc.

Using the Fox® System, researchers have advanced the understanding of therapeutic HOS by exploring epitope/paratope mapping, aggregation-interface identification, formulation, and small molecule binding. The Fox System, a novel HRPF technique, employs hydroxyl radicals (•OH) to irreversibly modify solvent-exposed amino acid side chains, revealing changes in solvent accessibility and uncovering buried or exposed regions. This presentation will discuss the Fox System, its application in characterizing biotherapeutic HOS and future innovations.

Presented by Dr. Emily Chea, Applied Research Manager of GenNext Technologies, Inc.

Mark Chance discussed his vision for proteomics and structural biology, focusing on megadalton protein complexes and the technologies needed to study them. He highlighted the importance of combining techniques like NMR, crystallography, cryo-EM, and mass spectrometry for protein interaction insights. He explained hydroxyl radical footprinting, a method for mapping protein surfaces and how it has advanced drug development. He shared examples from Rodeo Therapeutics, CASMA, and Foghorn Therapeutics, showcasing its practical applications.

Presented by Professor Mark Chance, Case Western Reserve University.

This 60-minute webinar, in collaboration with Cambridge Healthtech Institute, offers a comprehensive review and instruction in protein footprinting techniques and applications. Learn about various benchtop covalent modification methods and rapid labeling techniques like X-ray synchrotron and Fast Photo-Oxidation of Protein (FPOP) hydroxyl radical protein footprinting (HRPF). Explore their practical implications for biopharmaceutical research, protein interactions, biosimilar development, epitope and paratope mapping, ligand-binding, and conformational change analysis.

Presented by Professor Mark Chance of Case Western Reserve University and Professor Joshua Sharp of the University of Mississippi

Take a Deeper Dive

Literature

From poster notes with our latest data to product and technical literature, you can find what you’re looking for in the GenNext online literature library.

literature

Publications

Learn about Radical Protein Footprinting by reading the growing list of publications authored by GenNext customers and members of our Scientific Advisory Board.

publications

The Team

The GenNext team is an accomplished collection of experts who have been creating first-in-class life science tools for both startups and industry giants.

team

Why Partner with GenNext?

Hear from luminaries in structural biology research on why they decided to join the GenNext Technologies Scientific Advisory Board to help develop and advocate for Flash Oxidation (Fox) Hydroxyl Radical Protein Footprinting technology. These important partnerships underscore our commitment to advancing structural biology research and increasing the value of Fox technology to the biopharmaceutical industry.

Request a custom webinar for your lab.

Discover the Benefits of Radical Protein Footprinting

Accelerate the discovery and development of safer, more effective, and less costly biopharmaceuticals.

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