New & Featured Literature

Critical Use Cases of Fox® Protein Footprinting Technology in BioPharma

Fox® Technology excels in epitope mapping, aggregation analysis, biosimilarity assessment, and stability optimization while validating AI-generated drug discovery models in just days.

The Game-Changing AutoFox® System: Reinventing Protein Higher Order Structural Analysis

Comprised of a user-friendly benchtop instrument coupled with intuitive data analysis software, our second-generation instrument has evolved into a more robust and hands-free tool.

Literature on HRPF Technology, Products, and Services

Fox® Footprinting: The Next Generation of Protein HOS Analysis

Reinventing the study of protein Higher Order Structure (HOS) through automated Hydroxyl Radical Protein Footprinting (HRPF).

GenNext & Agilent Technology Integration

The integration of GenNext’s AutoFox Protein Footprinting System with Agilent’s 6560 Ion Mobility Quadrupole Time-of-Flight (IM-QTOF) LC/MS System presents an unparalleled approach to detailed protein structure analysis and biomolecular interaction studies.

Review of Technologies for Biopharmaceutical Higher Order Structural (HOS) Analysis

Identifying the most robust and state-of-the-art HOS analytical method for biotherapeutic discovery and development.

Frequently Asked Questions
Flash Oxidation (Fox) Protein Footprinting Services for Higher Order Structural (HOS) Analysis

Learn about HRPF services from what can be studied, what data can be generated, what samples are required, to starting a service project with GenNext.

Revolutionizing Protein Higher Order Structural Analysis

Novel products and services for Hydroxyl Radical Protein Footprinting (HRPF) to accelerate biopharmaceutical/biosimilar development and protein conformational research.

Unrivaled Higher Order Structural (HOS) Analysis for Biopharmaceutical Discovery & Development

Learn how your research programs will realize massive savings in time and money, robust and reproducible data generation and analysis, and a much simplified and faster workflow.

FoxWare® Protein Footprinting Software

Introducing new HRPF analysis software—a user-friendly bioinformatics tool for quickly and accurately interpreting results from Fast Photochemical Oxidation of Proteins (FPOP) experiments.

Evaluation of XIC Peaks for Inclusion in Average Peptide Oxidation Calculations

FoxWare Data Processing Software evaluates the XIC peaks to include them when calculating the average peptide oxidation.

Poster Notes & White Papers

Comparing an Epitope Identified from a Crystal Structure with In-Solution Mass Spectrometry-Based Techniques Including HRPF and XL-MS

HRPF and XL-MS provide complementary insights underscoring the importance of using multiple techniques for comprehensive protein characterization.

Demonstration of Adalimumab Structural Stability in Various pHs using High Throughput HRPF with the AutoFox® Protein Footprinting System

These findings provide valuable insights into the stability and conformational dynamics of Adalimumab under different pH conditions.

Streamline Mass Spec-Based Structural Biology Experiments with the High-Throughput AutoFox® Protein Footprinting System

The AutoFox System provides accurate measurements of solvent accessibility, as indicated by strong correlations between the calculated protection factors (PF) and known crystal structure solvent accessibility (SASA).

Automated Hydroxyl Radical Protein Footprinting in a 96-Well Format

First-in-class instrument for fully automated protein footprinting demonstrates highly precise and accurate quantification for numerous higher order structure (HOS) mapping experiments.

Improving TNFα’s Epitope Identification with Residue Level HRPF using the ZenoTOF 7600 system

Powerful MS/MS sensitivity and enhanced ion fragmentation technology allows for localization of challenging PTMs and more comprehensive understanding of protein structure.

Characterizing Glycan-Induced Structural Changes and Binding Interfaces for IgG1-C1q Complex using HRPF

HRPF identifies a binding pocket of IgG1 with C1Q with improved resolution using residues level analysis.

Revealing Antibody Aggregation with Fox® Protein Footprinting Technology

Understanding protein-protein interactions so researchers can develop high concentration biotherapeutics.

Improved Methods for Residue-Level Mass Spectrometry Data Acquisition and HRPF Data Interpretation

Learn how the inclusion list approach improved MS and low-intensity modified residue identification, along with improving matches by 30 times.

FoxWare® Software Resolves Isomeric Heterogeneity and Retention Time Drift in Oxidized Peptides from HRPF

Learn how FoxWare Software handles key issues with HRPF data analysis, increasing the statistical robustness of quantitation.

Laser-Free Flash Oxidation (Fox®) HRPF System Accurately Maps the Epitope of TNFα Bound to Adalimumab

Learn how Fox-based FPOP detected 4 TNF-α peptides with a significant decrease in oxidation following Ab binding known to be a part of the TNFα-Ab interface.

Characterizing Type III Kinase Inhibitor Using Laser-Free Flash Oxidation (Fox®) Hydroxyl Radical Protein Footprinting

Learn how the Fox HRPF System conducts In Vitro and In-cell FPOP to deliver a snapshot of the Higher Order Structure of the full proteome.

FoxWare^® Protein Footprinting Software Accurately Track Changes in Amino Acid Solvent Accessibility

Learn how FoxWare Software performs automated assignment and relative quantitation of residue-level changes in protein-binding interactions.

In Vitro and In-Cell Radical Dosimetry for Reproducible HRPF Experiments

Learn how the Fox®-based HRPF System conducts In Vitro and In-cell FPOP to deliver a snapshot of the Higher Order Structure of the full proteome.

FoxWare® Protein Footprinting Software — An Integrated Package for HRPF

Learn how FoxWare Software’s Radical Dosimetry, Data Processing, and Data Analysis Modules help you gain actionable insights from your HRPF experiments.