The Fox System can be employed for target identification to analyze protein samples from complex biological systems or mixtures. By subjecting the protein samples to hydroxyl radicals, Fox Footprinting can identify and distinguish target proteins based on their reactivity and protection against oxidation. This information helps in the initial identification and selection of potential target proteins for further investigation and validation.
To characterize protein interactions and study complex formation, the Fox System examines the changes in protein structure and solvent accessibility in the presence of interacting partners, identifying proteins that are involved in specific protein-protein interactions. This information aids in characterizing protein networks, signaling pathways, and potential drug targets.
Fox Footprinting can assist in validating the specificity of a potential drug target by subjecting protein samples to footprinting in the presence of a specific ligand or drug candidate, assessing the direct binding of the ligand to the target protein. Fox Technology can help confirm that the identified target is indeed affected by the ligand of interest.
The Fox System can contribute to the mapping of ligand binding sites on a target protein. By performing Fox Footprinting experiments in the presence and absence of a ligand or drug candidate, it is possible to identify the regions of the protein that are directly affected by the binding event. This information aids in understanding the ligand-target interaction and can guide the design of ligands with improved binding affinity and specificity.
Fox Technology can be employed to uncover insights into the functional regions and domains of a target protein. By subjecting the protein to footprinting, it is possible to identify regions that are critical for protein function or involved in specific interactions. This information aids in understanding the functional relevance of the target protein and its potential role in disease processes.