Fox System Accurately Maps the Paratope and Epitope of TNFα Bound to Adalimumab
ABSTRACT
A study on TNF alpha and its monoclonal antibody adalimumab, using the Fox Protein Footprinting System, revealed oxidation changes in TNF alpha peptides matching the known epitope. Adalimumab’s Fab region showed decreased oxidation due to reduced solvent accessibility when bound to TNF alpha, while the Fc region had increased oxidation, indicating flexibility beyond the crystal structure. This method accurately mapped the TNF alpha epitope and adalimumab paratope, providing insights beyond the crystal structure.
SPEAKER
Dr. Emily Chea received her PhD while studying with Professor Dr. Lisa Jones at the University of Maryland, Baltimore. Emily has made significant contributions to the development of HRPF, with particular emphasis on in-cell FPOP (IC-FPOP) for proteome-wide structural biology characterization of cellular drug–target interactions. Currently, Emily is a key member of the GenNext scientific team in her role as an Applied Research Scientist Manager.
