In-cell and in vivo FPOP
ABSTRACT
This hour-long seminar reviews recent advancements in in-cell and whole organism FPOP. It offers a detailed discussion on experimental design and format, highlighting the strengths and benefits of these methods compared to in vitro FPOP. The seminar also covers the implications for protein HOS research and drug development, featuring real research examples from eukaryotic cells and C. elegans.
SPEAKER
In the Department of Chemistry and Biochemistry at UCSD, the Jones Lab is a structural proteomics group that uses biochemical, analytical, and biophysical approaches to study protein interactions important in biological processes. Their research focuses on protein footprinting methods coupled with mass spectrometry to identify these interactions. A major focus of their lab is extending FPOP as an in-cell method for monitoring proteins in their native cellular environment. This method would be especially useful for membrane proteins, the largest class of drug targets, which are challenging to study in vitro owing to the difficulty of purifying these proteins. The Jones lab has further expanded FPOP for in vivo analysis in C. elegans, an animal model for human disease.
