Radical Protein Footprinting in Mammalian Whole Blood
ABSTRACT
Radical protein footprinting (RPF) is a structural biology technique used in cells and organisms. This presentation describes the first example of radical protein footprinting in mammalian blood using sulfate radicals. It shows the first oxidation of proteins by sodium persulfate using a Fox Protein Footprinting System, and the suitability of adenine dosimetry for measuring sulfate radical dose. The research also introduces improved mixing and quenching methods to reduce background oxidation, successfully applying RPF to stabilized whole mouse blood.
SPEAKER
Dr. Joshua Sharp is an internationally recognized expert in HRPF, pioneering novel MS-based technology to study HOS focusing on the structure-function relationships of proteins and carbohydrates. With more than 21 peer-reviewed articles, Dr. Sharp has also presented numerous invited lectures around the world. Along with acting as the Chief Technology Officer of GenNext, Dr. Sharp is also Associate Professor of Pharmacology, Associate Professor of Chemistry and Biochemistry, and Director of the Glycoscience Center of Research Excellence at the University of Mississippi.
