Meet Dr. Joshua Sharp

 Watch as Genny the GenNext Fox interviews the world-renowned and internationally recognized expert in Hydroxyl Radical Protein Footprinting (HRPF)—Dr. Joshua Sharp. Inventor of the first benchtop HRPF system and the pioneer of UV photolysis of hydrogen peroxide for hydroxyl radical generation, Josh has a lot of interesting things to say about HRPF.

jove Protocol

On-Demand Recording

Online review of method to perform flash oxidation protein footprinting using inline radical dosimetry and a plasma light source.

WATCH >

FASEB’s Protein Aggregation Conference:
Function, Dysfunction, and Disease

Flash Oxidation (FoxTM) Hydroxyl Radical Protein Footprinting for mAb Aggregation

On-Demand Recording

Spend less than 10-minutes with Dr. Chea as she reviews the Flash Oxidation (Fox™) Protein Footprinting System offered by GenNext Technologies. With Fox-based Hydroxyl Radical Protein Footprinting (HRPF), you can characterize monoclonal antibody (mAb) aggregation and determine how excipients help stabilize the protein. It has been established that excipients, like amino acids, can stabilize proteins and limit aggregation. By comparing the change in hydroxyl radical modification before and after the addition of an amino acid, you can detect intermolecular interactions that take place during mAb aggregation. By better understanding the higher order structure of protein aggregation, you will be armed with the information you need to optimize mAb formulation and development.

REGISTER/WATCH >
DOWNLOAD WHITE PAPER>

Presented by Emily Chea, PhD

Dr. Emily Chea received her PhD while studying with Professor Dr. Lisa Jones at the University of Maryland, Baltimore. Emily is a highly skilled research scientist in the use of LC-MS techniques for proteomics studies. Emily has made significant contributions to the development of FPOP and HRPF with particular emphasis towards in-cell FPOP (IC-FPOP). Emily focuses on utilizing IC-FPOP for proteome-wide structural biology to characterizing cellular drug–target interactions. Currently, Emily a key member of the GenNext scientific team in her role as Applied Research Scientist.

AAPS e-Chalk Talk

Revolutionizing Protein Higher Order Structural Analysis with Flash Oxidation (Fox) Protein Footprinting

Virtual Session
June 17, 2021
Thu 1:15 PM EDT

This chalk talk will offer an overview of Hydroxyl Radical Protein Footprinting, focusing on explaining the fundamentals of the technique and applications relevant to the pharmaceutical sciences. The foundations of hydroxyl radical protein labeling chemistry and the relationship of labeling to protein topography will be reviewed. Furthermore, a brief description of the Flash Oxidation (Fox) technology and the method used to generate radicals as well as the method for real-time dosimetry and the requirements for sample analysis by LC-MS/MS will be presented. Dr. Sharp will present examples of applications, focusing on the analysis of biopharmaceuticals, along with presenting data highlighting higher order structure effects of formulations. Dr. Sharp will show the effects of different buffers on the aggregation propensity and aggregation interfaces of a therapeutic monoclonal antibody along with verifying the ability of polysorbate to prevent aggregation without changing the topography of the monomeric protein. He will also present results defining the epitope of a therapeutic antibody. These applications illustrate a few of the potential applications of Fox technology towards the analysis of protein biopharmaceuticals.

REGISTER/WATCH >

Presented by Joshua Sharp, PhD

Dr. Joshua Sharp is a world-renowned and internationally recognized expert in Hydroxyl Radical Protein Footprinting (HRPF), pioneering some of the early work in studying protein structure and interactions. He has over twenty-one peer-reviewed articles and has presented numerous invited lectures on biopharmaceutical HRPF. Along with acting as the Chief Technology Officer of GenNext, Dr. Sharp is also Associate Professor of Pharmacology, Associate Professor of Chemistry and Biochemistry, and Director of the Glycoscience Center of Research Excellence at the University of Mississippi. Dr. Sharp is active in the development and application of new mass spectrometry-based technologzies for studying the structure-function relationships of proteins and carbohydrates, focusing on the study of interactions between neutralizing antibodies and the glycoprotein coat of HIV, characterization of chemokines and the structural factors mediating their oligomerization and function, and the identification of glycosaminoglycan structures.

ABRF 2021 Virtual Meeting

The Flash Oxidation (FoxTM) System: A Novel Laser-Free Photochemical Oxidation Protein Footprint Platform

Virtual Session
March 2021

Dr. Joshua Sharp, Chief Technology Officer of GenNext Technologies and Associate Professor of Pharmacology, Associate Professor of Chemistry and Biochemistry, and Director of the Glycoscience Center of Research Excellence at the University of Mississippi, presents during the ABRF 2021 Virtual Meeting on the Fox Footprinting System.

REGISTER/WATCH >

Request a custom HRPF webinar for your lab.

Request Webinar