Hydroxyl Radical Protein Footprinting Products & Services

GenNext has pioneered a superior, compact, cost-effective, and safe means of performing Hydroxyl Radical Protein Footprinting (HRPF) analysis by replacing expensive, complicated, and hazardous lasers with our proprietary Flash Oxidation (Fox®) Protein Footprinting System in a convenient benchtop format.

To address reproducibility challenges, GenNext has developed the Fox Radical Dosimeter — the world’s first OH radical measurement system that automatically controls the generation of effective OH radicals in the face of unpredictable and varying background scavenging. The Fox Dosimeter provides real-time measurement of OH radical concentration, making it simple to detect problems with biopharmaceutical solutions or assay conditions before mass spectrometric analysis is performed, vastly improving HRPF ease of use while simultaneously ensuring labeling confidence and fidelity.

To eradicate present-day bioinformatics and data processing bottlenecks, GenNext offers FoxWare® Data Processing — a unique data processing program specifically tailored to aptly address and automate data processing demands of HRPF HOS analysis.

Learn how our products can easily fit into your lab’s workflow or test-drive the Fox System on an outsourced project basis.

Hydroxyl Radical Protein Footprinting (HRPF) Services in Monoclonal Antibody Design & Production

HRPF is a powerful technique that is being used to generate high-value information for characterizing monoclonal antibodies (mAb) in the design and development of effective and safe biologics. Researchers are using this technique to study antigen-binding sites, epitope/paratope mapping, host antibody responses, antibody aggregation, and more.

In monoclonal antibody design, researchers are using the high-resolution structural information generated from HRPF for epitope and paratope mapping to accelerate the rational design of therapeutics. In monoclonal antibody production, researchers are using HRPF to monitor mAb structure during scale-up to ensure batch-to-batch structure stability over time, ensuring product shelf life and continued efficacy.

Unlike other protein structure analysis techniques, the Fox®Protein Footprinting System, which employs the HRPF approach, provides dynamic, in-solution structure and interaction information for a wide range of protein sizes, states, and concentrations.

Easily Explore the Advantages of HRPF Via Fox Services

Complement your traditional HOS methods or begin a totally new route to modern HOS analysis using GenNext’s services.

We’ve gathered everything you need to know about our contract services into a MONOCLONAL ANTIBODY TOOLKIT including presentations, papers, literature, and interviews. Just click the button below to learn how our exceptional service delivers new and actionable data beyond traditional HOS methods like MX, NMR, and cryoEM.

Announcing Partnership with Emery Pharma on Full-Solution Biopharmaceutical Protein Footprinting Services

We’re joining forces with Emery Pharma, a full-service CRO offering end-to-end biologic drug development support. Through our partnership, GenNext and Emery Pharma will provide best-in-class, full-solution pharmaceutical functional and structural analysis for allosteric and monoclonal antibody therapeutics. We will co-market our capabilities to clients seeking contract research programs for pharmaceutical and biopharmaceutical development. Together, we will deliver data-rich insights to help clients accelerate the approval of biotherapeutics, which will lead to safer, more effective, and less expensive therapeutic products.

Read the press release here.

Dr. Lisa Jones of UCSD to lead our R&D efforts into in vivo proteins as Cheif Science Officer

Dr. Lisa Jones, GenNext’s new Chief Science Officer (CSO), will lead the expansion of our Flash Oxidation (Fox) Protein Footprinting System into in vivo proteins. Under her direction, we are developing technology to study the protein interactions of membrane proteins, the largest class of drug targets. As director of the Jones Lab at UCSD, her team uses biochemical, analytical, and biophysical approaches coupled with mass spectrometry to identify protein interactions. Dr. Jones is the world-recognized pioneer of in-cell and whole-organism Fast Photochemical Oxidation of Proteins (FPOP). Read her JASMS profile article here.

Read our press release to see how GenNext is assembling a team that will make structural biology easier. And check out her recent ASMS cover article on the Development of Spheroid-FPOP: An In-Cell Protein Footprinting Method for 3D Tumor Spheroids.

Ron Hites Award for Outstanding Research Publication in JASMS

The Ron Hites Award—named to honor Professor Ron Hites of Indiana University who led the creation of JASMS in 1988—recognizes a high-quality presentation of outstanding original research. The yearly selection is based on the paper’s innovative aspects, technical quality, likely stimulation of future research, likely impact on future applications, and quality of presentation.

Joshua Sharp and coauthors Emily E. Chea, Sandeep K. Misra, Ron Orlando, Marla Popov, Robert W. Egan, David Holman, and Scot R. Weinberger earned this recognition for the paper entitled: “Flash Oxidation (FOX) System: A Novel Laser-Free Fast Photochemical Oxidation Protein Footprinting Platform.”

Read the award-winning JASMS article here.


On-Demand Presentations

Technologies for Higher
Order Structural Analysis

Helping you choose the method that empowers your lab to develop safe and effective biotherapeutics.

Fox™ Protein Footprinting
for mAb Aggregation

Learn how you can characterize mAb aggregation and determine how excipients help stabilize the protein.

A Novel Laser-Free
FPOP Platform

Overview of HRPF focusing on the fundamentals of the technique and applications in pharmaceutical science.

JoVE On-Line Protocol

Method to perform flash oxidation protein footprinting using inline radical dosimetry and a plasma light source.

Don’t Miss our 5-Part Protein Footprinting Seminar Series!


The application of Fox-based HRPF technology in biopharmaceutical research is always expanding, from drug-binding, mAb therapeutics, and protein aggregation to formulation studies.


Watch recorded presentations, protocols, and interviews with GenNext scientists and our Scientific Advisory Board on on subjects ranging from applications to methods.

HOS Technology

GenNext sifted through the available HOS analysis options to help you determine the most robust, cost-effective, and workflow-friendly approach for your lab.

The Team

The GenNext team is an accomplished collection experts who have been creating first-in-class life science tools for both startups and industry giants.


Learn about Hydroxyl Radical Protein Footprinting technology by reading the growing list of publications authored by GenNext scientists and members of our Scientific Advisory Board.

Meet the GenNext Scientific Advisory Board

Meet Professor David Tabb

Hear what Dr. Tabb from the Institut Pasteur has to say about key innovations in hardware and software that have transformed proteomics. And learn why revealing biological information with the right tools, removing roadblocks caused by black boxes, and continued bioinformatics education are important themes in his research. Then, he discusses why he joined the Scientific Advisory Board to help better understand the datasets produced by GenNext systems that probe protein structure.

Meet Professor Lisa Jones

Professor Lisa Jones invented in-cell and in-vivo Fast Photo-Oxidation of Proteins (FPOP) to study cells and more complex model systems that could not be analyzed by Hydrogen Deuterium Exchange. Taking advantage of irreversible labeling, Lisa’s lab began to use FPOP to study disease pathogenesis in a new way. Most recently, her team is using FPOP to elucidate protein aggregation in different disease states.

Meet Professor Michael Gross

Listen to Professor Michael Gross as he describes the invention of Fast Photo-Oxidation of Proteins (FPOP) for Hydroxyl Radical Protein Footprinting (HRPF). Professor Gross highlights how it is being applied in epitope mapping studies. He anticipates a great future for footprinting in antibody drug development for improved biotherapeutics.

Meet Dr. Mark Chance

Meet the world-famous father of HRPF, Dr. Mark Chance. In this interview, he describes being motivated to develop a new technique to see, with much greater resolution, proteins in motion. Living in the “magical middle ground” of moderate cost and higher resolution, Dr. Chance discusses his lab’s use HRPF technology to study neurological samples to detect the severity of a debilitating disease.

Meet Dr. Joshua Sharp

Watch as Genny™ the GenNext Fox interviews the internationally recognized expert in Hydroxyl Radical Protein Footprinting (HRPF)—Dr. Joshua Sharp. As the inventor of the first benchtop HRPF system and the pioneer of UV photolysis of hydrogen peroxide for hydroxyl radical generation, Josh has a lot of interesting things to say about the current success and future potential of protein footprinting.

Request a custom HRPF webinar for your lab.

Discover the Benefits of HRPF

Accelerate biopharmaceutical and biosimilar development and protein conformational research.