Modern Analysis of Biotherapeutic
Structure & Interaction
State-of-the-Art Protein Footprinting Instruments & Services
Our novel Flash Oxidation (Fox®) Protein Footprinting Technology employs hydroxyl radicals (•OH) to examine the solvent accessibility of amino acid side chains within a protein. By selectively labeling exposed amino acids, •OH radicals induce the formation of oxidation products, enabling the detection and analysis of vital information concerning protein structure and dynamics.
Unlike other techniques, Fox Protein Footprinting delivers dynamic, in-solution structure and interaction information on a wide range of protein sizes, states, and concentrations—crucial information that can be used to shape the safety, efficacy, and quality of biologics.
This powerful, new structural biology technology helps researchers
navigate the complexity of discovering and developing world-class biotherapeutics.
Direct Assessment of Solvent Accessibility
Higher-Order Structure Validation
High Spatial Resolution
Versatility Across Protein Classes
Complementary to Other Techniques
Protein-Protein Interactions
Epitope & Paratope Mapping
Protein-Ligand Interactions
Protein Folding & Conformational Changes
Regions Impacted by Allostery
Glycoprotein Structure & Function
- Small Molecule Therapeutic Target Engagement & Response
Rapidly Validate AI-Based Structural Models
Fox Protein Footprinting Services
Fast, Precise, and Actionable Insights
GenNext specializes in using our proprietary Fox Protein Footprinting platform to answer your most immediate structural biology questions—quickly, accurately, and cost-effectively. We can work with a wide variety of molecules, including mAbs, ADCs, multispecifics, cytokines, Fc-fusions, and other complex protein systems.
To accelerate insights while minimizing upfront cost and risk, consider GenNext as your research partner. Our turnkey, fee-for-service program delivers high-precision results through a streamlined, expert-led process.
Hands-Free, High-Throughput
& Reproducible Labeling
& Reproducible Labeling
First Fully-Automated Radical Protein Footprinting
With the AutoFox® Protein Footprinting System, GenNext enables biopharmaceutical researchers to perform high-resolution protein footprinting studies with robustness, reproducibility, and ease—generating high-value structural insights in days instead of weeks or months.
GenNext’s FoxWare® Software removes data processing bottlenecks with this powerful program tailored to meet the demands of HOS analysis.
Learn how our products can easily fit into your lab’s workflow, or test-drive the Fox System on an outsourced project basis.
Applications from Discovery to Development
Insights from Biosimilars to AI Model Validation
Delivering Answers in a New Way
The Fox Technology platform pairs an intuitive workflow with the robust FoxWare Protein Footprinting Software, producing results that rival even the most advanced, technically demanding, and expensive methods in structural biology. This integrated approach streamlines complex analyses, making top-tier structural data attainable for a wider range of laboratories. By minimizing operational complexity and lowering costs, the system empowers researchers to gain detailed insights into protein structures and dynamics with greater efficiency and ease than ever before.
See how the Fox platform stacks up against other structural biology methods.
GenNext News
NEW PUBLICATION: Recommendations and Considerations for Hydroxyl Radical Protein Footprinting–Mass Spectrometry
A recent study published in Nature Methods examines the full HRPF workflow from experimental design and sample preparation through data analysis.
Our CTO Josh Sharp, along with Scientific Advisory Board members Lisa Jones and Michael Gross, joined a prestigious group of colleagues from academia and industry to publish this in-depth review of Hydroxyl Radical Protein Footprinting.
This guide walks through the key best practices covering sample preparation, data analysis, and how to combine footprinting results with other structural methods.
Learn how to perform the technique confidently whether you're in an academic lab or a biopharma setting.
NEW PUBLICATION: Radical Footprinting in Mammalian Whole Blood
A new study in Nature Communications demonstrates something that, until now, simply wasn’t possible—measuring protein conformational changes and surface accessibility directly in mammalian whole blood.
Our CTO, Josh Sharp, and his colleagues studied detailed structural changes at the residue level in a complex biological setting without having to separate or disturb the system. By capturing disease-associated conformational changes in a T2DM model, the study validated that meaningful biological signals can be measured in situ. This represents a shift from studying proteins in isolation... to studying them as they exist and behave in real biological systems.
Enormous congrats to Josh and his colleagues for proving that GenNext’s Radical Protein Footprinting can move beyond the limitations of traditional research methods to understand proteins in their native biological context.
Take a Deeper Dive
Literature
From poster notes with our latest data to product and technical literature, you can find what you’re looking for in the GenNext online literature library.
Publications
Learn about Radical Protein Footprinting by reading the growing list of publications authored by GenNext customers and members of our Scientific Advisory Board.
The Team
The GenNext team is an accomplished collection of experts who have been creating first-in-class life science tools for both startups and industry giants.
Why Partner with GenNext?
Hear from luminaries in structural biology research on why they decided to join the GenNext Technologies Scientific Advisory Board to help develop and advocate for Flash Oxidation (Fox) Hydroxyl Radical Protein Footprinting technology. These important partnerships underscore our commitment to advancing structural biology research and increasing the value of Fox technology to the biopharmaceutical industry.
Discover the Benefits of Radical Protein Footprinting
Accelerate the discovery and development of safer, more effective, and less costly biopharmaceuticals.