Hydroxyl Radical Protein Footprinting Products & Services

GenNext has pioneered a superior, compact, cost-effective, and safe means of performing Hydroxyl Radical Protein Footprinting (HRPF) analysis by replacing expensive, complicated, and hazardous lasers with our proprietary Flash Oxidation (Fox™) Protein Footprinting System in a convenient benchtop format.

To address reproducibility challenges, GenNext has developed the Fox Radical Dosimeter — the world’s first OH radical measurement system that automatically controls the generation of effective OH radicals in the face of unpredictable and varying background scavenging. The Fox Dosimeter provides real-time measurement of OH radical concentration, making it simple to detect problems with biopharmaceutical solutions or assay conditions before mass spectrometric analysis is performed, vastly improving HRPF ease of use while simultaneously ensuring labeling confidence and fidelity.

To eradicate present day bioinformatics and data processing bottlenecks, GenNext introduces FoxWare™ Data Processing — a unique data processing program specifically tailored to aptly address and automate data processing demands of HRPF HOS analysis.

Learn how our products can easily fit into your lab’s workflow or test-drive the Fox System on an outsourced project basis.

Flash Oxidation (Fox™) Protein Footprinting System

Dr. Lisa Jones of UCSD to lead our R&D efforts into in vivo proteins as Cheif Science Officer

Dr. Lisa Jones, GenNext’s new Chief Science Officer (CSO), will lead the expansion of our Flash Oxidation (Fox™) Protein Footprinting System into in vivo proteins. Under her direction, we are developing technology to study the protein interactions of membrane proteins, the largest class of drug targets. As director of the Jones Lab at UCSD, her team uses biochemical, analytical, and biophysical approaches coupled with mass spectrometry to identify protein interactions. Dr. Jones is the world-recognized pioneer of in-cell and whole-organism Fast Photochemical Oxidation of Proteins (FPOP). Read her JASMS profile article here.

Read our press release to see how GenNext is assembling a team that will make structural biology easier.

On-Demand Presentations

Technologies for Higher
Order Structural Analysis

Helping you choose the method that empowers your lab to develop safe and effective biotherapeutics.

Fox™ Protein Footprinting
for mAb Aggregation

Learn how you can characterize mAb aggregation and determine how excipients help stabilize the protein.

A Novel Laser-Free
FPOP Platform

Overview of HRPF focusing on the fundamentals of the technique and applications in pharmaceutical science.

JoVE On-Line Protocol

Method to perform flash oxidation protein footprinting using inline radical dosimetry and a plasma light source.

Don’t Miss our 5-Part Protein Footprinting Seminar Series!


The application of Fox™-based HRPF technology in biopharmaceutical discovery and development is always expanding, from drug-binding, mAb therapeutics, and protein aggregation to formulation studies.

HOS Technology

GenNext sifted through the available HOS analysis options, from low- to high-resolution approaches, to help you determine the most robust, cost-effective, and workflow-friendly approach for your lab.


Watch recorded presentations, protocols, and interviews with GenNext scientists and our Scientific Advisory Board on Fox™-based HRPF on subjects ranging from applications to methods.

The Team

The GenNext team is an accomplished collection of scientific, engineering, and commercialization experts who have been creating first-in-class life science tools for both startups and industry giants.


Learn about Hydroxyl Radical Protein Footprinting technology by reading the growing list of publications authored by GenNext scientists and members of our Scientific Advisory Board.

NIH Support

Research and Development efforts at GenNext have been graciously supported by NIH Small Business Innovation and Research Awards from the National Institute of General Medical Sciences.

Meet the GenNext Scientific Advisory Board

Meet Professor David Tabb

Hear what Dr. Tabb from the Institut Pasteur has to say about key innovations in hardware and software that have transformed proteomics. And learn why revealing biological information with the right tools, removing roadblocks caused by black boxes, and continued bioinformatics education are important themes in his research. Then, he discusses why he joined the Scientific Advisory Board to help better understand the datasets produced by GenNext systems that probe protein structure.

Meet Professor Lisa Jones

Professor Lisa Jones invented in-cell and in-vivo Fast Photo-Oxidation of Proteins (FPOP) to study cells and more complex model systems that could not be analyzed by Hydrogen Deuterium Exchange. Taking advantage of irreversible labeling, Lisa’s lab began to use FPOP to study disease pathogenesis in a new way. Most recently, her team is using FPOP to elucidate protein aggregation in different disease states.

Meet Professor Michael Gross

Listen to Professor Michael Gross as he describes the invention of Fast Photo-Oxidation of Proteins (FPOP) for Hydroxyl Radical Protein Footprinting (HRPF). Professor Gross highlights how it is being applied in epitope mapping studies. He anticipates a great future for footprinting in antibody drug development for improved biotherapeutics.

Meet Dr. Mark Chance

Meet the world-famous father of HRPF, Dr. Mark Chance. In this interview, he describes being motivated to develop a new technique to see, with much greater resolution, proteins in motion. Living in the “magical middle ground” of moderate cost and higher resolution, Dr. Chance discusses his lab’s use HRPF technology to study neurological samples to detect the severity of a debilitating disease.

Meet Dr. Joshua Sharp

Watch as Genny™ the GenNext Fox interviews the internationally recognized expert in Hydroxyl Radical Protein Footprinting (HRPF)—Dr. Joshua Sharp. As the inventor of the first benchtop HRPF system and the pioneer of UV photolysis of hydrogen peroxide for hydroxyl radical generation, Josh has a lot of interesting things to say about the current success and future potential of protein footprinting.

Request a custom HRPF webinar for your lab.

Discover the Benefits of HRPF

Accelerate biopharmaceutical and biosimilar development and protein conformational research.