Monoclonal Antibody Therapeutic Characterization using Flash Oxidation (Fox®) Protein Footprinting Technology

Presented by Emily Chea, GenNext Technologies

Utilizing the Fox System, researchers have explored the effects of epitope/paratope mapping, aggregation-interface identification, formulation, small molecule binding, and other factors, thereby enhancing the understanding and significance of therapeutic HOS. The Fox System is a novel HRPF technique that utilizes hydroxyl radicals (•OH) to irreversibly modify solvent-exposed amino acid side chains. As amino acids’ solvent accessibility changes, the •OH modification concordantly changes. This uncovers regions buried or exposed after changing conditions, down to the amino acid resolution. This presentation will provide background on the Fox System, how it is being applied to characterize biotherapeutic HOS, and future advancements to the Fox System innovation.