Structural Biology at Amino Acid Resolution: Integrating AutoFox® HRPF with Multiple High-Resolution MS Platforms
ABSTRACT
The GenNext AutoFox® Protein Footprinting System with mass spectrometry enables amino acid-level mapping of protein interactions and conformational changes. Advanced MS tools like the Sciex ZenoTOF 7600 and Agilent 6560 address challenges include fragment ion identification for accurate oxidation localization and isobaric peptide co-elution causing chimeric spectra. Furthermore, integrating Thermo’s XL-MS further resolves ambiguous signals, advancing biologics and biotherapeutics research.
SPEAKER
Dr. Emily Chea received her PhD while studying with Professor Dr. Lisa Jones at the University of Maryland, Baltimore. Emily has made significant contributions to the development of HRPF, with particular emphasis on in-cell FPOP (IC-FPOP) for proteome-wide structural biology characterization of cellular drug–target interactions. Currently, Emily is a key member of the GenNext scientific team in her role as an Applied Research Scientist Manager.
