Recommendations and Considerations for Hydroxyl Radical Protein Footprinting–Mass Spectrometry
Wecksler, A.T., Wang, L., Bernstein, L.J. et al.; Nat Methods (May 2026) ABSTRACT Protein oxidative footprinting, using hydroxyl radical labeling detected by bottom-up proteomics, has progressed from an emerging method to a widely used approach in structural biology. Hydroxyl radicals generated from hydrogen peroxide (via photolysis, Fenton chemistry or electrochemistry) or directly from water (via X-rays, [...]